Purification and characterization of glutathione-dependent dehydroascorbate reductase from rat liver

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Purification and characterization of glutathione-dependent dehydroascorbate reductase from rat liver.

GSH-dependent enzymic reduction of dehydroascorbic acid to ascorbic acid has been studied in rat liver cytosol. After gel filtration of cytosol on Sephadex G-100 SF, dehydroascorbate reductase activity was recovered in two distinct peaks, one corresponding to glutaredoxin (an enzyme already known for its dehydroascorbate reductase activity) and another, much larger one, corresponding to a novel...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1994

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj3010471